Apostolos K. Kiritsakis


School of Food Technology and Nutrition, Technological Educational Institution of Thessaloniki, Sindos Thessaloniki, Greece





The purpose of this paper is to describe the factors affecting olive oil composition and to overview the effect of its composition on nutrition and human health. It is well accepted that the high mono-unsaturation of olive oil and the presence of several other constituents such as phenols and tocopherols, chlorophyll and pheophytin, sterols, squalene, aroma and flavor compounds and other exhibit a significant role on the health. Olive oil as a highly monounsaturated oil, is resistant to oxidation. Also the presence of phenols, tocopherols and other natural antioxidants prevent lipid oxidation within the body eliminating the formation of free radicals which may cause cell destruction. The aroma and the flavor compounds of olive oil, as well as, the chlorophyll and pheophytin pigments increase the stomach secretion and facilitate the absorption of the natural antioxidants, which furthermore protect the body tissues from oxidation. Epidemiological studies suggest that the high consumption of the monounsaturated olive oil in Mediterranean countries, is related with the low rates of cardiovascular disease (CHD), cancer of the breast and of high life expectancy.


KEYWORDS: phenols, radicals, monounsaturation, aging, aroma compounds, HDL cholesterol, assimilation




Olive oil contains triacylglycerols and small quantities of free fatty acids, glycerol, pigments, aroma compounds, sterols, tocopherols, phenols, unidentified resinous components and other (Kiritsakis, 1998). Among these constituents the unsaponifiable fraction (Fig. 1), which covers a small percentage (0,5-1,5 %) plays a significant role on human health.


1.1 Oleic acid


Olive oil contains a high percentage of the monounsaturated oleic acid. Thus, it is  a natural monounsaturated oil. This particular fatty acid reduces LDL-cholesterol, which is responsible for the formation of the atherosclerotic plaque, and increases the HDL- cholesterol. The latter is removed from arterial cells.


1.2    Hydrocarbon squalene


The  hydrocarbon  squalene,  an  important  metabolic  factor, is  present  in high

percentages in olive oil (Fig.1).


1.3  Tocopherols


Olive oil contains the tocopherols α-, β-, γ-, δ- (α-tocopherol covers almost  88%). The tocopherol content of olive oil depends not only on the presence of these compounds in olive fruit but also on several other factors, involved in the transportation, storage and olive fruit processing. According to Viola (1997), the ratio of vitamin-E to polyunsaturated fatty acids in olive oil is better than to other edible oils.


Figure 1: Unsaponifiable components of olive oil.


1.4   Pigments


The color of olive oil is mainly related to the presence of chlorophyll and pheophytin. Carotenoids  are also responsible for the color of olive oil. The presence of these constituents depend on several factors, such as cultivar, soil and climate, fruit maturation as well as applied  conditions during olive fruit processing.


1.5  Phenolic compounds


Olive fruit contains simple and complex phenolic compounds. Most of these compounds are passing into the oil, increase its oxidative stability and  improve the  taste. Hydrohytyrosol, tyrosol and some phenolic acids are mainly found  in olive oil (Kiritsakis,1998). The phenol content and the specific composition of these phenols  in olive oil  depend on the altitude where the olive trees are grown, on the  harvesting time and on the processing conditions  (Cinquanta et al.,1997; Kiritsakis, 1998).


1.6 Aroma components

Aroma and taste of olive oil are its main sensory characteristics. These characteristics are attributed  to  a group of aroma compounds. Their formation occurs in olive fruit, via a series of enzymatic reactions (Kiritsakis,1998). Figure 2 shows a gas chromatography mass spectroscopy (GC-MS) analysis of  three different olive oil  samples. In the sample of good quality (a), trans-2-hexenal is the predominant component. The other two samples (b, c) have defects (Tateo et al.,1993).


Figure 2: Gas chromatography mass spectroscopy analysis of the flavor components of three samples of olive oil (a: fruity flavor, b and c: with defects) (Tateo et al., 1993).

Olive oil is greatly assimilated by the human body. The assimilation of this “natural fruit juice” by our body is mainly attributed to the  high percentage of  triolein. Also, the pigments chlorophyll and pheophytin and  the aroma components present, facilitate its absorption from the human body. The latter differentiate the gastric fluid composition of the stomach and increase the digestive activity. The great assimilation of olive oil from the human body facilitates  the absorption of vitamin-E and phenols as well.
The accumulation of free radicals, as a result of oxidation in the body, causes serious problems on human health. More specific, free radicals destroy the polyunsaturated fatty acids of the membranes and the DNA (Fig. 3), which facilitates the aging process, causes damage to the liver and even cancer formation. Our body is protected from the free radicals by free radicals scavengers such as, vitamin-E and  phenols. The latter, present in significant amounts in olive oil, prevents the human’s cell destruction. Greeks and Italians, who consume large quantities of virgin olive oil, intake almost 25 gr of phenols per day, which is a significant amount for preventing oxidation.

Figure 3: Effect of free radicals in polyunsaturated fatty acids of membranes and DNA. (Viola, 1997).




Recent studies showed that LDL-cholesterol oxidation promotes the atherosclerotic plaque formation (Kafatos, 1995; Lenart et al., 1998). Diets rich in olive oil on the other hand showed a stable ratio between total cholesterol and HDL. and  a reduction  of LDL-cholesterol.  Even though the role of triacylglycerols  in coronary disease is still unclarrified, the substitution of olive oil with complex starches in the diet, decreases the triacylglycerols in blood serum. Greece, a member of the seven country study with the highest consumption of olive oil, shows the lowest number of deaths from coronary disease (Trichopoulou, 1993). Resent studies in South Italy, showed an increase in cholesterol content almost 2% every year, after the abundance of Mediterranean diet, in which olive oil is the major fat component.




In conclusion, oleic acid and mainly the unsaponifiable fraction of olive oil, such as phenols, tocopherols, chlorophyll, squalene and aroma components, exhibit a high nutritional and biological value, resulting in good human health.





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Kiritsakis, A. Olive oil- Second Edition, From the tree to the table. Food and Nutrition Press, Inc., Trumbull, Connecticut, 006611, USA  (1998).


Lenart, E.B., Willet, W., and Kiritsakis, A.,Ibid.


Tateo, E., Brunelli, N., Cucurachi, S., and Ferrillo, A.,. New trends in the study of the merits and shortcomings of olive oil in organoleptic terms, in correlation with the GC/MS analysis of the aromas. In Food Flavors, Ingredients and Composition, G. Charalampous, editor,  Elsevier Science Publishers B.V., Amsterdam (1993).


Trichopoulou, A., Katsoyanni K., and Gnardellis, C., The traditional Greek diet. European Journal of Clinical Nutrition (Suppl 1). 47:76 (1993).


Viola, P., Olive oil and Health. International Olive Oil Council, Madrid Spain (1997).